Metabolomic Study of Aging in fa/fa Rats: Multiplatform Urine and Serum Analysis

0575321 - MBÚ 2024 RIV CH eng J - Journal Article
Pelantová, Helena - Tomášová, Petra - Šedivá, B. - Neprašová, Barbora - Mráziková, Lucia - Kuneš, Jaroslav - Železná, Blanka - Maletínská, Lenka - Kuzma, Marek
Metabolomic Study of Aging in fa/fa Rats: Multiplatform Urine and Serum Analysis.
Metabolites. Roč. 13, č. 4 (2023), č. článku 552. E-ISSN 2218-1989
R&D Projects: GA ČR(CZ) GA20-00546S
Institutional support: RVO:61388971 ; RVO:61388963
Keywords : fa/fa rats * genetic obesity * metabolomics * nmr * lc-ms
OECD category: Biochemistry and molecular biology; Physiology (including cytology) (UOCHB-X)
Impact factor: 4.1, year: 2022
Method of publishing: Open access
https://www.mdpi.com/2218-1989/13/4/552

Zucker fatty (fa/fa) rats represent a well-established and widely used model of genetic obesity. Because previous metabolomic studies have only been published for young fa/fa rats up to 20 weeks of age, which can be considered early maturity in male fa/fa rats, the aim of our work was to extend the metabolomic characterization to significantly older animals. Therefore, the urinary profiles of obese fa/fa rats and their lean controls were monitored using untargeted NMR metabolomics between 12 and 40 weeks of age. At the end of the experiment, the rats were also characterized by NMR and LC-MS serum analysis, which was supplemented by a targeted LC-MS analysis of serum bile acids and neurotransmitters. The urine analysis showed that most of the characteristic differences detected in young obese fa/fa rats persisted throughout the experiment, primarily through a decrease in microbial co-metabolite levels, the upregulation of the citrate cycle, and changes in nicotinamide metabolism compared with the age-related controls. The serum of 40-week-old obese rats showed a reduction in several bile acid conjugates and an increase in serotonin. Our study demonstrated that the fa/fa model of genetic obesity is stable up to 40 weeks of age and is therefore suitable for long-term experiments.
Permanent Link: https://hdl.handle.net/11104/0345113