Three-dimensional diamond structures for co-culture tissue engineering

0575297 - FZÚ 2024 RIV SK eng C - Conference Paper (international conference)
Potocký, Štěpán - Szabó, Ondrej - Staňková, Ľubica - Zehetner, J. - Dohnal, F. - Bačáková, Lucie - Kromka, Alexander
Three-dimensional diamond structures for co-culture tissue engineering.
Proceedings of ADEPT - ADEPT 2023. Žilina: University of Žilina, 2023 - (Jandura, D.; Lettrichová, I.; Kováč, jr., J.), s. 206-209. ISBN 978-80-554-1977-0.
[International Conference on Advances in Electronic and Photonic Technologies /11./ - ADEPT 2023. Podbanské (SK), 12.06.2023-15.06.2023]
R&D Projects: GA MŠMT LM2023051; GA MŠMT(CZ) EF16_019/0000760
Grant - others:AV ČR(CZ) SAV-23-12; OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760
Program: Bilaterální spolupráce
Institutional support: RVO:68378271 ; RVO:67985823
Keywords : diamond * CVD * mesenchymal cells * endothelial cells * viability
OECD category: Materials engineering; Biomaterials (as related to medical implants, devices, sensors) (FGU-C)

The successful fabrication of diamond-coated three-dimensional substrates is a base for creating highly vascularized bone tissue. The F, H, and O diamond surface terminations were tested against monocultures of human mesenchymal and endothelial cells as well as their co-culture. The cell numbers and viabilities show that O termination of a diamond is the best option for cell growth with respect to other terminations. On the first day of cultivation, the number of hMSC significantly exceeded the number on the control with the viability of almost 100 %. Similar results were also achieved for hMSCs where the co-culture significantly exceeded the cell number on the control. On the third day, the number of hMSCs remained significantly the highest, even against the control. On the seventh day of cultivation, both cell types formed a confluent layer.
Permanent Link: https://hdl.handle.net/11104/0345075