Genosensing on a 3D-printed nanocarbon electrode

0575169 - BFÚ 2024 RIV NL eng J - Journal Article
Jyoti, A. - Fojta, Miroslav - Hermanová, Monika - Pivoňková, Hana - Alduhaish, O. - Pumera, M.
Genosensing on a 3D-printed nanocarbon electrode.
Electrochemistry Communications. Roč. 151, JUN 2023 (2023), č. článku 107508. ISSN 1388-2481. E-ISSN 1873-1902
R&D Projects: GA ČR(CZ) GA20-03187S
Institutional support: RVO:68081707
Keywords : 3D-printed nanocarbon electrode * DNA hybridization * Electrochemical analysis * Additive manufacturing
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Impact factor: 5.4, year: 2022
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S1388248123000826?via%3Dihub

In this paper we present the characterization of 3D-printed nanocarbon electrodes (3DnCes) and their application in electrochemical enzyme-linked detection of DNA hybridization. The approach takes advantage of a facile procedure based on adsorption of target DNA on the electrode surface followed by hybridization with a biotinylated probe and binding of streptavidin-alkaline phosphatase conjugate. The alkaline phosphatase converts 1-naphthyl phosphate in the background electrolyte into electrochemically oxidizable 1-naphthol, which is subsequently detected using linear sweep voltammetry. The preparation, characterization, and analytical performance of the 3DnCes are reported. The results show the applicability of such 3DnCes in detection of target DNA hybridization specifically with the complementary biotinylated probe, and indicate the potential of 3D printed electrodes for use in various bioanalytical approaches.
Permanent Link: https://hdl.handle.net/11104/0349247