Development of recombinant West Nile virus expressing mCherry reporter protein

0574469 - BC 2024 RIV NL eng J - Journal Article
Kobayashi, S. - Fukuda, Y. - Yoshii, K. - Thammahakin, P. - Maezono, K. - Eyer, Luděk - Růžek, Daniel - Kariwa, H.
Development of recombinant West Nile virus expressing mCherry reporter protein.
Journal of Virological Methods. Roč. 317, APR (2023), č. článku 114744. ISSN 0166-0934. E-ISSN 1879-0984
Institutional support: RVO:60077344
Keywords : West Nile virus * Reporter protein * Production of reporter virus
OECD category: Biochemistry and molecular biology
Impact factor: 3.1, year: 2022
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0166093423000691?via%3Dihub

West Nile virus (WNV) is transmitted to humans and animals by a mosquito and enters the central nervous system, leading to lethal encephalitis. Reporter viruses expressing fluorescent proteins enable detection of infected cells in vitro and in vivo, facilitating evaluation of the dynamics of viral infection, and the development of diagnostic or therapeutic methods. In this study, we developed a method for production of a recombinant replication-competent WNV expressing mCherry fluorescent protein. The expression of mCherry was observed in viral antigen-positive cells in vitro and in vivo, but the growth of the reporter WNV was reduced as compared to the parental WNV. The expression of mCherry was stable during 5 passages in reporter WNV-infected culture cells. Neurological symptoms were observed in mice inoculated intracranially with the reporter WNV. The reporter WNV expressing mCherry will facilitate research into WNV replication in mouse brains.
Permanent Link: https://hdl.handle.net/11104/0345586