Comprehensive ECG reference intervals in C57BL/6N substrains provide a generalizable guide for cardiac electrophysiology studies in mice

0574410 - ÚMG 2024 RIV DE eng J - Journal Article
Oestereicher, M. A. - Wotton, J. M. - Ayabe, S. - Bou About, G. - Cheng, T. K. - Choi, J. - Clary, D. - Dew, E. M. - Elfertak, L. - Guimond, A. - Haseli Mashhadi, H. - Heaney, J. D. - Kelsey, L. - Keskivali-Bond, P. - Lopez Gomez, F. - Marschall, S. - McFarland, M. - Meziane, H. - Munoz Fuentes, V. - Nam, K. - Nichtová, Zuzana - Pimm, D. - Bower, L. - Procházka, Jan - Rozman, Jan - Santos, L. - Stewart, M. - Tanaka, N. - Ward, Ch. S. - Willett, A. M. E. - Sedláček, Radislav … Total 51 authors
Comprehensive ECG reference intervals in C57BL/6N substrains provide a generalizable guide for cardiac electrophysiology studies in mice.
Mammalian Genome. Roč. 34, č. 2 (2023), s. 180-199. ISSN 0938-8990. E-ISSN 1432-1777
R&D Projects: GA MŠMT ED2.1.00/19.0395; GA MŠMT(CZ) LM2015040; GA MŠMT(CZ) LM2018126; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT EF16_013/0001789; GA MŠMT EF18_046/0015861
Institutional support: RVO:68378050
Keywords : Electrocardiography * Electrophysiologic Techniques * Inbred C57BL * C57BL mouse * heart function test
OECD category: Biochemistry and molecular biology
Impact factor: 2.5, year: 2022
Method of publishing: Open access
https://link.springer.com/article/10.1007/s00335-023-09995-y

Reference ranges provide a powerful tool for diagnostic decision-making in clinical medicine and are enormously valuable for understanding normality in pre-clinical scientific research that uses in vivo models. As yet, there are no published reference ranges for electrocardiography (ECG) in the laboratory mouse. The first mouse-specific reference ranges for the assessment of electrical conduction are reported herein generated from an ECG dataset of unprecedented scale. International Mouse Phenotyping Consortium data from over 26,000 conscious or anesthetized C57BL/6N wildtype control mice were stratified by sex and age to develop robust ECG reference ranges. Interesting findings include that heart rate and key elements from the ECG waveform (RR-, PR-, ST-, QT-interval, QT corrected, and QRS complex) demonstrate minimal sexual dimorphism. As expected, anesthesia induces a decrease in heart rate and was shown for both inhalation (isoflurane) and injectable (tribromoethanol) anesthesia. In the absence of pharmacological, environmental, or genetic challenges, we did not observe major age-related ECG changes in C57BL/6N-inbred mice as the differences in the reference ranges of 12-week-old compared to 62-week-old mice were negligible. The generalizability of the C57BL/6N substrain reference ranges was demonstrated by comparison with ECG data from a wide range of non-IMPC studies. The close overlap in data from a wide range of mouse strains suggests that the C57BL/6N-based reference ranges can be used as a robust and comprehensive indicator of normality. We report a unique ECG reference resource of fundamental importance for any experimental study of cardiac function in mice.
Permanent Link: https://hdl.handle.net/11104/0344738