Thermoneutral housing promotes hepatic steatosis in standard diet-fed C57BL/6N mice, with a less pronounced effect on NAFLD progression upon high-fat feeding

0574364 - FGÚ 2024 RIV CH eng J - Journal Article
Horáková, Olga - Sistilli, Gabriella - Kalendová, Veronika - Bardová, Kristina - Mitrović, Marko - Čajka, Tomáš - Irodenko, Ilaria - Janovská, Petra - Lackner, K. - Kopecký, Jan - Rossmeisl, Martin
Thermoneutral housing promotes hepatic steatosis in standard diet-fed C57BL/6N mice, with a less pronounced effect on NAFLD progression upon high-fat feeding.
Frontiers in Endocrinology. Roč. 14, Jul 12 (2023), č. článku 1205703. ISSN 1664-2392. E-ISSN 1664-2392
R&D Projects: GA MZd(CZ) NU21-01-00469; GA MŠMT(CZ) LX22NPO5104
EU Projects: European Commission(XE) 722619 - Foie Gras
Institutional support: RVO:67985823
Keywords : non-alcoholic fatty liver disease * liver steatosis * NASH * C57BL/6N mice * obesity * thermoneutrality * metabolomics * de novo lipogenesis
OECD category: Endocrinology and metabolism (including diabetes, hormones)
Impact factor: 5.2, year: 2022
Method of publishing: Open access
https://doi.org/10.3389/fendo.2023.1205703

Introduction: Non-alcoholic fatty liver disease (NAFLD) can progress to more severe stages, such as steatohepatitis and fibrosis. Thermoneutral housing together with high-fat diet promoted NAFLD progression in C57BL/6J mice. Due to possible differences in steatohepatitis development between different C57BL/6 substrains, we examined how thermoneutrality affects NAFLD progression in C57BL/6N mice.
Methods: Male mice were fed standard or high-fat diet for 24 weeks and housed under standard (22°C) or thermoneutral (30°C) conditions.
Results: High-fat feeding promoted weight gain and hepatic steatosis, but the effect of thermoneutral environment was not evident. Liver expression of inflammatory markers was increased, with a modest and inconsistent effect of thermoneutral housing, however, histological scores of inflammation and fibrosis were generally low (<1.0), regardless of ambient temperature. In standard diet-fed mice, thermoneutrality increased weight gain, adiposity, and hepatic steatosis, accompanied by elevated de novo lipogenesis and changes in liver metabolome characterized by complex decreases in phospholipids and metabolites involved in urea cycle and oxidative stress defense.
Conclusion: Thermoneutrality appears to promote NAFLD-associated phenotypes depending on the C57BL/6 substrain and/or the amount of dietary fat.

Permanent Link: https://hdl.handle.net/11104/0344701