Iron oxide nanoparticles trigger endoplasmic reticulum damage in steatotic hepatic cells

0574215 - FZÚ 2024 RIV GB eng J - Journal Article
Uzhytchak, Mariia - Lunova, Mariia - Smolková, Barbora - Jirsa, M. - Dejneka, Alexandr - Lunov, Oleg
Iron oxide nanoparticles trigger endoplasmic reticulum damage in steatotic hepatic cells.
Nanoscale Advances. Roč. 5, č. 16 (2023), s. 4250-4268. ISSN 2516-0230. E-ISSN 2516-0230
R&D Projects: GA MŠMT(CZ) EF16_019/0000760
Grant - others:OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760
Institutional support: RVO:68378271
Keywords : iron oxide nanoparticles * magnetic resonance imaging * cytotoxicity * hepatic steatosis * ER stress * apoptosis
OECD category: Biophysics
Impact factor: 4.7, year: 2022
Method of publishing: Open access

Iron oxide nanoparticles (IONPs) are being actively researched in various biomedical applications, particularly as magnetic resonance imaging contrast agents for diagnosing various liver pathologies. Emerging evidence suggests that IONPs may exacerbate hepatic steatosis and liver injury in susceptible livers. However, our understanding of how IONPs may affect steatotic cells at the sub-cellular level is still fragmented. In this study, we demonstrate that IONPs, at a dose that does not cause general toxicity in hepatic cells, induce significant toxicity in steatotic cells. Mechanistically, co-treatment with palmitic acid and IONPs resulted in endoplasmic reticulum (ER) stress, accompanied by the release of cathepsin B from lysosomes to the cytosol. The release of cathepsin B, along with ER stress, led to the activation of apoptotic cell death. This study provides important basic knowledge for the future optimization of IONPs as MRI contrast agents for various biomedical applications.
Permanent Link: https://hdl.handle.net/11104/0344559