Hydrophilic copolymers with hydroxamic acid groups as a protective biocompatible coating of maghemite nanoparticles: synthesis, physico-chemical characterization and MRI biodistribution study

0573957 - ÚMCH 2024 RIV CH eng J - Journal Article
Charvátová, Hana - Plichta, Zdeněk - Hromádková, Jiřina - Herynek, V. - Babič, Michal
Hydrophilic copolymers with hydroxamic acid groups as a protective biocompatible coating of maghemite nanoparticles: synthesis, physico-chemical characterization and MRI biodistribution study.
Pharmaceutics. Roč. 15, č. 7 (2023), č. článku 1982. E-ISSN 1999-4923
R&D Projects: GA MŠMT LX22NPO5102
Research Infrastructure: Czech-BioImaging III - 90250
Institutional support: RVO:61389013
Keywords : superparamagnetic iron oxide nanoparticles * maghemite * hydroxamic acid
OECD category: Polymer science
Impact factor: 5.4, year: 2022
Method of publishing: Open access
https://www.mdpi.com/1999-4923/15/7/1982

Superparamagnetic iron oxide nanoparticles (SPION) with a “non-fouling” surface represent a versatile group of biocompatible nanomaterials valuable for medical diagnostics, including oncology. In our study we present a synthesis of novel maghemite (γ-Fe2O3) nanoparticles with positive and negative overall surface charge and their coating by copolymer P(HPMA-co-HAO) prepared by RAFT (reversible addition–fragmentation chain-transfer) copolymerization of N-(2-hydroxypropyl)methacrylamide (HPMA) with N-[2-(hydroxyamino)-2-oxo-ethyl]-2-methyl-prop-2-enamide (HAO). Coating was realized via hydroxamic acid groups of the HAO comonomer units with a strong affinity to maghemite. Dynamic light scattering (DLS) demonstrated high colloidal stability of the coated particles in a wide pH range, high ionic strength, and the presence of phosphate buffer (PBS) and serum albumin (BSE). Transmission electron microscopy (TEM) images show a narrow size distribution and spheroid shape. Alternative coatings were prepared by copolymerization of HPMA with methyl 2-(2-methylprop-2-enoylamino)acetate (MMA) and further post-polymerization modification with hydroxamic acid groups, carboxylic acid and primary-amino functionalities. Nevertheless, their colloidal stability was worse in comparison with P(HPMA-co-HAO). Additionally, P(HPMA-co-HAO)-coated nanoparticles were subjected to a bio-distribution study in mice. They were cleared from the blood stream by the liver relatively slowly, and their half-life in the liver depended on their charge. Nevertheless, both cationic and anionic particles revealed a much shorter metabolic clearance rate than that of commercially available ferucarbotran.

Permanent Link: https://hdl.handle.net/11104/0344378