Column selection for improvement of the UHPLC lipidomic analysis

0573205 - ÚOCHB 2024 RIV DE eng A - Abstract
Vrkoslav, Vladimír - Pražáková, Kateřina - Strnad, Štěpán - Sýkora, D. - Cvačka, Josef
Column selection for improvement of the UHPLC lipidomic analysis.
HPLC 2023. 51st International Symposium on High-Performance Liquid Phase Separations and Related Techniques. Düsseldorf: German Chemical Society, 2023.
[HPLC 2023. International Symposium on High-Performance Liquid Phase Separations and Related Techniques /51./. 18.06.2023-22.06.2023, Düsseldorf]
R&D Projects: GA MŠMT(CZ) LX22NPO5104
Institutional support: RVO:61388963
Keywords : UHPLC/MS * lipidomic strategy
OECD category: Analytical chemistry

Reversed-phase UHPLC/MS is a widely used lipidomic strategy that allows the separation of the lipid species based on their equivalent carbon number (ECN - the number of carbons minus two times the number of double bonds of all fatty acids). Lipid classes as well as fatty acid compositions are determined using fragments in MSn spectra. Retention time has to correspond with identifying lipid species, so the knowledge of retention dependences significantly enhances the certainty of identification. Increasing chromatographic peak resolution allows identifying more lipid species, and short run times are essential for analyzing large sets of samples in lipidomics. Reducing the slope of the gradient is not a suitable solution for increasing resolution, so selecting a suitable stationary phase is critical. In this study, four C18 columns with sub-2-micrometer particles were compared using a mixture of 71 lipid standards from 16 lipid classes.

Permanent Link: https://hdl.handle.net/11104/0343658