Effect of heparin as an anticoagulant on measurements of the erythrocyte genome size using flow cytometry in bony fishes of different ploidy

0573126 - ÚBO 2024 RIV CZ eng J - Journal Article
Halačka, Karel - Janko, K. - Fedorčák, J. - Koščo, J. - Vetešník, Lukáš
Effect of heparin as an anticoagulant on measurements of the erythrocyte genome size using flow cytometry in bony fishes of different ploidy.
Acta veterinaria Brno. Roč. 92, č. 2 (2023), s. 171-179. ISSN 0001-7213. E-ISSN 1801-7576
Institutional support: RVO:68081766
Keywords : C-value * dapi * DNA content * Fish * Propidium iodide
OECD category: Veterinary science
Impact factor: 0.6, year: 2022
Method of publishing: Open access
https://actavet.vfu.cz/media/pdf/actavet_2023092020171.pdf

The aim of the study was to verify whether the use of heparin as an anticoagulant can affect the results of genome size measurements using a flow cytometer. The values obtained by measuring blood samples with different concentrations of heparin were compared. The differences observed were highly significant, suggesting that the results of previous comparative studies, or those determining individual polyploidy, may need reinterpretation. We anticipate that our findings will increase the accuracy of flow cytometric analysis and will be useful for further surveys of genome size undertaken using blood samples.
Permanent Link: https://hdl.handle.net/11104/0343594