Interaction of lectin Sambucus nigra with sialylated trisaccharides in the presence of osmolytes. Chronopotentiometric sensing

0573065 - BFÚ 2024 RIV NL eng J - Journal Article
Galicová, Tatiana - Hasoň, Stanislav - Ostatná, Veronika
Interaction of lectin Sambucus nigra with sialylated trisaccharides in the presence of osmolytes. Chronopotentiometric sensing.
Bioelectrochemistry. Roč. 152, AUG 2023 (2023), č. článku 108457. ISSN 1567-5394. E-ISSN 1878-562X
R&D Projects: GA ČR(CZ) GA22-26590S
Institutional support: RVO:68081707
Keywords : Lectin SNA-1 interactions * Osmolytes * Chronopotentiometric stripping * Impedance Ct curves * Sialylated trisaccharides * Water arrangement
OECD category: Biochemistry and molecular biology
Impact factor: 5, year: 2022
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S1567539423000944?via%3Dihub

Trisaccharides bind to their interaction partners-lectins relatively weakly, which makes detection of their complexes challenging. In this work, we show that an osmolyte presence improves the distinguishing complexes of lectin Sambucus nigra with trisialyllactoses with various binding affinities. The addition of osmolyte, non-binding sugar mannose significantly improved the precision of binding experiments performed using chro-nopotentiometric stripping at the electrode surface and fluorescence analysis in solution. Osmolytes minimized nonspecific interactions between binding sugar and lectin. Obtained findings can be utilized in any in vitro methods studying interactions of carbohydrates, respectively their conjugates with proteins. The study of car-bohydrate interactions appears important since they play essential roles in a variety of biological processes including carcinogenesis.
Permanent Link: https://hdl.handle.net/11104/0350038