First insight in element localisation in different body parts of the acanthocephalan Dentitruncus truttae using TEM and NanoSIMS

0573063 - BC 2024 RIV NL eng J - Journal Article
Marijic, V. F. - Subirana, M. A. - Schaumloeffel, D. - Barisic, J. - Gontier, E. - Krasnici, N. - Mijosek, T. - Hernández-Orts, Jesús S. - Scholz, Tomáš - Erk, M.
First insight in element localisation in different body parts of the acanthocephalan Dentitruncus truttae using TEM and NanoSIMS.
Science of the Total Environment. Roč. 887, AUG (2023), č. článku 164010. ISSN 0048-9697. E-ISSN 1879-1026
R&D Projects: GA ČR(CZ) GX19-28399X
Institutional support: RVO:60077344
Keywords : Nanoscale secondary ion mass spectrometry * Transmission electron microscopy * Correlative imaging * Thorny-headed worms * Body ultrastructure * Chemical composition
OECD category: Marine biology, freshwater biology, limnology
Impact factor: 9.8, year: 2022
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0048969723026311?via%3Dihub

Acanthocephalans, intestinal parasites of vertebrates, are characterised by orders of magnitude higher metal accumu-lation than free-living organisms, but the mechanism of such effective metal accumulation is still unknown. The aim of our study was to gain new insights into the high-resolution localization of elements in the bodies of acanthocephalans, thus taking an initial step towards elucidating metal uptake and accumulation in organisms under real environmental conditions. For the first time, nanoscale secondary ion mass spectrometry (NanoSIMS) was used for high-resolution mapping of 12 elements (C, Ca, Cu, Fe, N, Na, O, P, Pb, S, Se, and Tl) in three selected body parts (trunk spines, inner part of the proboscis receptacle and inner surface of the tegument) of Dentitruncus truttae, a parasite of brown trout (Salmo trutta) from the Krka River in Croatia. In addition, the same body parts were examined using transmission electron microscopy (TEM) and correlated with NanoSIMS images. Metal concentrations determined using HR ICP-MS confirmed higher accumulation in D. truttae than in the fish intestine. The chemical composition of the acanthoceph-alan body showed the highest density of C, Ca, N, Na, O, S, as important and constitutive elements in living cells in all studied structures, while Fe was predominant among trace elements. In general, higher element density was found in trunk spines and tegument, as body structures responsible for substance absorption in parasites. The results obtained with NanoSIMS and TEM-NanoSIMS correlative imaging represent pilot data for mapping of elements at nanoscale res-olution in the ultrastructure of various body parts of acanthocephalans and generally provide a contribution for further application of this technique in all parasite species.
Permanent Link: https://hdl.handle.net/11104/0343718