Development of the Pseudomonas syringae pv. morsprunorum Biofilm Monitored in Real Time Using Attenuated Total Reflection Fourier Transform Infrared Measurements in a Flow Cell Chamber

0572880 - FZÚ 2024 RIV GB eng J - Journal Article
Budil, Jakub - Štenclová, Pavla - Kromka, Alexander - Lišková, P.
Development of the Pseudomonas syringae pv. morsprunorum Biofilm Monitored in Real Time Using Attenuated Total Reflection Fourier Transform Infrared Measurements in a Flow Cell Chamber.
Applied Spectroscopy. Roč. 77, č. 5 (2023), s. 500-512. ISSN 0003-7028. E-ISSN 1943-3530
R&D Projects: GA MŠMT LM2023051; GA MŠMT(CZ) EF16_019/0000760
Grant - others:OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760
Research Infrastructure: e-INFRA CZ - 90140
Institutional support: RVO:68378271
Keywords : ATR FT-IR * infrared attenuated total reflection * IR-ATR * Pseudomonas syringae * biofilm * confocal laser scanning microscopy * CLSM * model
OECD category: Microbiology
Impact factor: 3.5, year: 2022
Method of publishing: Limited access
https://doi.org/10.1177/00037028231165057

BIn the present study, infrared attenuated total reflection spectroscopy coupled with optical and confocal laser scanning microscopy has been applied for the first time to analyze Pseudomonas syringae pathovar morsprunorum biofilm development in real time. The biofilm development was observed within a spectral window 4000–800 cm−1 under constant flow conditions for 72 h. The kinetics of representative integrated band areas (nucleic acids with polysaccharides at 1141–1006 cm−1, amino acid side chains with free fatty acids at 1420–1380 cm−1, proteins at 1580–1490 cm−1, and lipids with proteins at 2935–2915 cm−1) were analyzed with regard to the observed biofilm structure and the following P. syringae biofilm developmental stages were attributed: The inoculation phase, washing of weakly attached bacteria closely followed by recolonization of the vacated surface, the restructuration phase, and finally the maturation phase.
Permanent Link: https://hdl.handle.net/11104/0350531