Raman imaging monitors the time-resolved response of A. thaliana to the artificial inhibition of PSII

0572826 - ÚVGZ 2024 RIV GB eng J - Journal Article
Vítek, Petr - Klem, Karel
Raman imaging monitors the time-resolved response of A. thaliana to the artificial inhibition of PSII.
Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. Roč. 291, APR (2023), č. článku 122276. ISSN 1386-1425. E-ISSN 1873-3557
R&D Projects: GA MŠMT(CZ) EF16_019/0000797
Research Infrastructure: CzeCOS IV - 90248
Institutional support: RVO:86652079
Keywords : Arabidopsis thaliana * Bioimaging * Light stress * Phenotyping * Raman spectrometry * Repressor of silencing
OECD category: Agronomy, plant breeding and plant protection
Impact factor: 4.4, year: 2022
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S138614252201424X

The short-term (0–96 h) response of A. thaliana to the oxidative stress induced by PSII inhibitor metribuzin was examined using Raman spectroscopy. Whole leaves of wildtype (WT, Col-0) and ros1 mutant were scanned and changes in carotenoids were examined. Strong differences in Raman intensity distributions between WT and ros1 were observed. A stronger decrease of carotenoid v1(C=C) band intensity across the leaf was observed in ros1 after 48 h of exposure to metribuzin. It can be assumed that higher sensitivity to oxidative stress in ros1 mutant results in significantly faster degradation of carotenoids.
Permanent Link: https://hdl.handle.net/11104/0343387