Advances in separation and identification of biologically importantmilk proteins and peptides

0572699 - ÚIACH 2025 RIV US eng J - Journal Article
Šťastná, Miroslava
Advances in separation and identification of biologically importantmilk proteins and peptides.
Electrophoresis. Roč. 45, 1-2 (2024), s. 101-119. ISSN 0173-0835. E-ISSN 1522-2683
R&D Projects: GA ČR(CZ) GA23-04703S
Institutional support: RVO:68081715
Keywords : MS-based proteomics * bioactive proteins/peptides * separation methods
OECD category: Analytical chemistry
Impact factor: 2.9, year: 2022
Method of publishing: Limited access

Milk is a rich source of biologically important proteins and peptides. In addition, milk contains a variety of extracellular vesicles (EVs), including exosomes, that carry their own proteome cargo. EVs are essential for cell-cell communication and modulation of biological processes. They act as nature carriers of bioactive proteins/peptides in targeted delivery during various physiological and pathological conditions. Identification of the proteins and protein-derived peptides in milk and EVs and recognition of their biological activities and functions had a tremendous impact on food industry, medicine research, and clinical applications. Advanced separation methods, mass spectrometry (MS)-based proteomic approaches and innovative biostatistical procedures allowed for characterization of milk protein isoforms, genetic/splice variants, posttranslational modifications and their key roles, and contributed to novel discoveries. This review article discusses recently published developments in separation and identification of bioactive proteins/peptides from milk and milk EVs, including MS-based proteomic approaches.
Permanent Link: https://hdl.handle.net/11104/0343306