Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: In Vivo Post-Grafting Safety Characterization in Rats and Adult Pig

0572489 - ÚŽFG 2024 RIV GB eng J - Journal Article
Kobayashi, Y. - Shigyo, M. - Platoshyn, O. - Marsala, S. - Kato, T. - Takamura, N. - Yoshida, K. - Kishino, A. - Bravo-Hernandez, M. - Juhás, Štefan - Juhásová, Jana - Studenovská, Hana - Proks, Vladimír - Driscoll, S. P. - Glenn, T. D. - Pfaff, S. L. - Ciacci, J. D. - Marsala, M.
Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: In Vivo Post-Grafting Safety Characterization in Rats and Adult Pig.
Cell Transplantation. Roč. 32, č. 1 (2023), č. článku 09636897221107009. ISSN 0963-6897. E-ISSN 1555-3892
Institutional support: RVO:67985904 ; RVO:61389013
Keywords : human induced pluripotent stem cells * neural precursor cells * cryopreservation
OECD category: Cell biology; Polymer science (UMCH-V)
Impact factor: 3.3, year: 2022
Method of publishing: Open access
https://journals.sagepub.com/doi/10.1177/09636897221107009

One of the challenges in clinical translation of cell-replacement therapies is the definition of optimal cell generation and storage/recovery protocols which would permit a rapid preparation of cell-treatment products for patient administration. Besides, the availability of injection devices that are simple to use is critical for potential future dissemination of any spinally targeted cell-replacement therapy into general medical practice. Here, we compared the engraftment properties of established human-induced pluripotent stem cells (hiPSCs)-derived neural precursor cell (NPCs) line once cells were harvested fresh from the cell culture or previously frozen and then grafted into striata or spinal cord of the immunodeficient rat. A newly developed human spinal injection device equipped with a spinal cord pulsation-cancelation magnetic needle was also tested for its safety in an adult immunosuppressed pig. Previously frozen NPCs showed similar post-grafting survival and differentiation profile as was seen for freshly harvested cells. Testing of human injection device showed acceptable safety with no detectable surgical procedure or spinal NPCs injection-related side effects.
Permanent Link: https://hdl.handle.net/11104/0343146