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An Enzymatic Biosensor with Amperometric Detection in a Flow Injection Analysis for the Determination of L-lactic Acid: Development and Application
- 1.0572488 - ÚFCH JH 2025 RIV CZ eng C - Conference Paper (international conference)
Tvorynska, Sofiia - Barek, J. - Josypčuk, Bohdan
An Enzymatic Biosensor with Amperometric Detection in a Flow Injection Analysis for the Determination of L-lactic Acid: Development and Application.
Moderní elektrochemické metody /42./. Sborník přednášek. Ústí nad Labem: Best servis, 2023 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), s. 213-217. ISBN 978-80-908947-0-9.
[Moderní elektrochemické metody /42./. Jetřichovice (CZ), 22.05.2023-26.05.2023]
R&D Projects: GA ČR(CZ) GA20-07350S
Institutional support: RVO:61388955
Keywords : L-lactic acid * Lactate oxidase * Amperometric biosensor * silver amalgam
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
An amperometric biosensor consisting of an enzymatic mini-reactor (lactate oxidase covalently
attached to −NH2 functionalized mesoporous silica powder SBA−15 using glutaraldehyde) and
a silver amalgam-based screen-printed electrode acting as a transducer was developed for the
determination of L-lactic acid (LA) in FIA. The detection potential of −0.9 V vs. Ag pseudoreference
electrode was applied for cathodic detection of enzymatically consumed oxygen.
Under the optimized conditions, the constructed biosensor enabled selective determination of
LA with a micromolar limit of detection. Importantly, the proposed biosensor represented
excellent operational stability after ≥350 measurements. Finally, it was successfully applied to
real sample analysis.
Permanent Link: https://hdl.handle.net/11104/0343145
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