Profiling of adrenal corticosteroids in blood and local tissues of mice during chronic stress

0572442 - FGÚ 2024 RIV US eng J - Journal Article
Vagnerová, Karla - Jágr, M. - Mekadim, Chahrazed - Ergang, Peter - Sechovcová, Hana - Vodička, Martin - Olša Fliegerová, Kateřina - Dvořáček, V. - Mrázek, Jakub - Pácha, Jiří
Profiling of adrenal corticosteroids in blood and local tissues of mice during chronic stress.
Scientific Reports. Roč. 13, č. 1 (2023), č. článku 7278. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA ČR(CZ) GA21-10845S
Institutional support: RVO:67985823 ; RVO:67985904
Keywords : microbiome * adrenal cortex hormones * stress
OECD category: Physiology (including cytology); Microbiology (UZFG-Y)
Impact factor: 4.6, year: 2022
Method of publishing: Open access
https://doi.org/10.1038/s41598-023-34395-2

Stress increases plasma concentrations of corticosteroids, however, their tissue levels are unclear. Using a repeated social defeat paradigm, we examined the impact of chronic stress on tissue levels of corticosterone (CORT), progesterone (PROG), 11-deoxycorticosterone (11DOC) and 11-dehydrocorticosterone (11DHC) and on gut microbiota, which may reshape the stress response. Male BALB/c mice, liquid chromatography-tandem mass spectrometry and 16S RNA gene sequencing were used to screen steroid levels and fecal microbiome, respectively. Stress induced greater increase of CORT in the brain, liver, and kidney than in the colon and lymphoid organs, whereas 11DHC was the highest in the colon, liver and kidney and much lower in the brain and lymphoid organs. The CORT/11DHC ratio in plasma was similar to the brain but much lower in other organs. Stress also altered tissue levels of PROG and 11DOC and the PROG/11DOC ratio was much higher in lymphoid organs that in plasma and other organs. Stress impacted the β- but not the α-diversity of the gut microbiota and LEfSe analysis revealed several biomarkers associated with stress treatment. Our data indicate that social defeat stress modulates gut microbiota diversity and induces tissue-dependent changes in local levels of corticosteroids, which often do not reflect their systemic levels.
Permanent Link: https://hdl.handle.net/11104/0343092