Mechanical regulation of mitochondrial dynamics and function in a 3D-engineered liver tumor microenvironment

0572095 - FZÚ 2024 RIV US eng J - Journal Article
Frtús, Adam - Smolková, Barbora - Uzhytchak, Mariia - Lunova, Mariia - Jirsa, M. - Petrenko, Yuriy - Dejneka, Alexandr - Lunov, Oleg
Mechanical regulation of mitochondrial dynamics and function in a 3D-engineered liver tumor microenvironment.
ACS BIOMATERIALS SCIENCE & ENGINEERING. Roč. 9, č. 5 (2023), s. 2408-2425. ISSN 2373-9878. E-ISSN 2373-9878
R&D Projects: GA ČR(CZ) GA22-18634S
Institutional support: RVO:68378271 ; RVO:68378041
Keywords : engineered cell microenvironments * cancer * mitochondria * mechanical forces * extracellular matrix * cytoskeleton * cell plasticity
OECD category: Biophysics; Biophysics (UEM-P)
Impact factor: 5.8, year: 2022
Method of publishing: Open access

It has become evident that physical stimuli of the cellular microenvironment transmit mechanical cues regulating key cellular functions, such as proliferation, migration, and malignant transformation. Accumulating evidence suggests that tumor cells face variable mechanical stimuli that may induce metabolic rewiring of tumor cells. We therefore designed soft 3D collagen scaffolds mimicking a pathological mechanical environment to decipher how liver tumor cells would adapt their metabolic activity to physical stimuli of the cellular microenvironment. Here, we report that the soft 3D microenvironment upregulates the glycolysis of HepG2 and Alexander cells. Both cell lines adapt their mitochondrial activity and function under growth in the soft 3D microenvironment.
Permanent Link: https://hdl.handle.net/11104/0342923