The Influence of Calcium on the Growth, Morphology and Gene Regulation in Gemmatimonas phototrophica

0571792 - MBÚ 2024 RIV CH eng J - Journal Article
Shivaramu, Sahana - Tomasch, Jurgen - Kopejtka, Karel - Nupur, Nupur - Saini, Mohit Kumar - Bokhari, Syed Nadeem Hussain - Küpper, Hendrik - Koblížek, Michal
The Influence of Calcium on the Growth, Morphology and Gene Regulation in Gemmatimonas phototrophica.
Microorganisms. Roč. 11, č. 1 (2023), č. článku 27. E-ISSN 2076-2607
R&D Projects: GA ČR(CZ) GX19-28778X; GA MŠMT EF15_003/0000336
Institutional support: RVO:61388971 ; RVO:60077344
Keywords : Gemmatimonas phototrophica * anoxygenic phototrophic bacteria * horizontal gene transfer * calcium * transcriptomics
OECD category: Microbiology; Microbiology (BC-A)
Impact factor: 4.5, year: 2022
Method of publishing: Open access
https://www.mdpi.com/2076-2607/11/1/27

The bacterium Gemmatimonas phototrophica AP64 isolated from a freshwater lake in the western Gobi Desert represents the first phototrophic member of the bacterial phylum Gemmatimonadota. This strain was originally cultured on agar plates because it did not grow in liquid medium. In contrast, the closely related species G. groenlandica TET16 grows both on solid and in liquid media. Here, we show that the growth of G. phototrophica in liquid medium can be induced by supplementing the medium with 20 mg CaCl2 L-1. When grown at a lower concentration of calcium (2 mg CaCl2 L-1) in the liquid medium, the growth was significantly delayed, cells were elongated and lacked flagella. The elevated requirement for calcium is relatively specific as it can be partially substituted by strontium, but not by magnesium. The transcriptome analysis documented that several groups of genes involved in flagella biosynthesis and transport of transition metals were co-activated after amendment of 20 mg CaCl2 L-1 to the medium. The presented results document that G. phototrophica requires a higher concentration of calcium for its metabolism and growth compared to other Gemmatimonas species.
Permanent Link: https://hdl.handle.net/11104/0342691