Oxidative stress and DNA fragmentation in frozen/thawed common Carp Cyprinus carpio sperm with and without supplemental proteins

0571674 - MBÚ 2024 RIV NL eng J - Journal Article
Shaliutina-Loginova, A. - Loginov, Dmitry Sergej
Oxidative stress and DNA fragmentation in frozen/thawed common Carp Cyprinus carpio sperm with and without supplemental proteins.
Animal Reproduction Science. Roč. 251, April 2023 (2023), č. článku 107213. ISSN 0378-4320. E-ISSN 1873-2232
Research Infrastructure: CENAKVA - 90099
Institutional support: RVO:61388971
Keywords : Cyprinidae * Freeze * thaw * Proteins * Reactive oxygen species * Sperm DNA fragmentation
OECD category: Biochemistry and molecular biology
Impact factor: 2.2, year: 2022
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0378432023000271?via%3Dihub

Using cryopreservation techniques can increase the effectiveness of reproducing cultured fish species by ensuring a dependable supply of sperm, although the quality of the sperm could be impacted by the procedures involved. The goal of this study was to investigate the effect of pu-rified seminal plasma transferrin (Tf), bovine serum albumin (BSA), and antifreeze protein (AFP) types I and III at 1 mu g mL-1 on relevant characteristics of cryopreserved sperm from common carp Cyprinus carpio. We compared oxidative stress indices, antioxidant activity, and DNA fragmen-tation of fresh sperm to that frozen with extender only or with Tf, BSA, or AFP types I and III. Fresh sperm had significantly lower levels of thiobarbituric acid reactive substances (TBARS) compared to samples that underwent cryopreservation without protein treatment, which resulted in 0.54 +/- 0.06 nmol/108 cells of TBARS. Carbonyl derivatives of proteins (CP) decreased significantly (ANOVA, P > 0.05) in carp sperm with addition of Tf, AFPI, and AFPIII. Significant differences in superoxide dismutase (SOD), glutathione reductase (GR), and glutathione peroxi-dase (GPx) activity were seen in sperm supplemented with Tf, BSA, AFPI, and AFPIII from those without. Significantly less DNA damage, expressed as percent tail DNA (11.56 +/- 1.34) and olive tail moment (0.59 +/- 0.13), was recorded in samples cryopreserved with Tf. The findings indi-cated that addition of Tf, BSA, AFPI, or AFPIII to cryopreservation medium is beneficial to sperm preservation. The mechanisms through which these proteins act positively on sperm need to be further investigated.
Permanent Link: https://hdl.handle.net/11104/0342846