Anti-Restriction Activity of ArdB Protein against EcoAI Endonuclease

0571669 - MBÚ 2024 RIV RU eng J - Journal Article
Kudryavtseva, A. A. - Alekhin, V. A. - Lebedeva, M. D. - Cséfalvay, Eva - Weiserová, Marie - Manukhov, I. V.
Anti-Restriction Activity of ArdB Protein against EcoAI Endonuclease.
Molecular Biology. Roč. 57, č. 1 (2023), s. 101-104. ISSN 0026-8933. E-ISSN 1608-3245
Institutional support: RVO:61388971
Keywords : antirestriction * rm-i * ArdB * EcoAI
OECD category: Microbiology
Impact factor: 1.2, year: 2022
Method of publishing: Limited access
https://link.springer.com/article/10.1134/S0026893323010053

ArdB proteins are known to inhibit the activity of the type I restriction-modification (RM-I) system, in particular EcoKI (IA family). The mechanism of ArdB's activity still remains unknown, the spectrum of targets inhibited has been poorly studied. In this work, it was shown that the presence of the ardB gene from the R64 plasmid could suppress the activity of EcoAI endonuclease (IB family) in Escherichia coli TG1 cells. Due to the absence of specificity of ArdB to a certain RM-I system (it inhibits both the IA- and IB-family), it can be assumed that the mechanism of the anti-restriction activity of this protein does not depend on the sequence DNA at the recognition site nor the structure of the restriction enzyme of the RM-I systems.
Permanent Link: https://hdl.handle.net/11104/0342848