Protection of nitrogenase from photosynthetic O2 evolution in Trichodesmium: methodological pitfalls and advances over 30 years of research

0571462 - MBÚ 2024 RIV CZ eng J - Journal Article
Hania, Anxhela - Lopez-Adams, Rebeca - Prášil, Ondřej - Eichner, Meri
Protection of nitrogenase from photosynthetic O2 evolution in Trichodesmium: methodological pitfalls and advances over 30 years of research.
Photosynthetica. Roč. 61, č. 1 (2023), s. 58-72. ISSN 0300-3604. E-ISSN 1573-9058
R&D Projects: GA ČR(CZ) GJ20-02827Y; GA ČR(CZ) GA20-17627S; GA MŠMT(CZ) EF20_079/0017812
Institutional support: RVO:61388971
Keywords : cyanobacteria * diazocyte * immunolabelling * method comparison * microscopy * O2-scavenging mechanisms
OECD category: Microbiology
Impact factor: 2.7, year: 2022
Method of publishing: Open access
https://ps.ueb.cas.cz/artkey/phs-202301-0006_protection-of-nitrogenase-from-photosynthetic-o2-evolution-in-trichodesmium-methodological-pitfalls-and-advan.php

The Trichodesmium genus comprises some of the most abundant N2-fixing organisms in oligotrophic marine ecosystems. Since nitrogenase, the key enzyme for N2 fixation, is irreversibly inhibited upon O2 exposure, these organisms have to coordinate their N2-fixing ability with simultaneous photosynthetic O2 production. Although being the principal object of many laboratory and field studies, the overall process of how Trichodesmium reconciles these two mutually exclusive processes remains unresolved. This is in part due to contradictory results that fuel the Trichodesmium enigma. In this review, we sift through methodological details that could potentially explain the discrepancy between findings related to Trichodesmium's physiology. In doing so, we exhaustively contrast studies concerning both spatial and temporal nitrogenase protective strategies, with particular attention to more recent insights. Finally, we suggest new experimental approaches for solving the complex orchestration of N2 fixation and photosynthesis in Trichodesmium.
Permanent Link: https://hdl.handle.net/11104/0342680