Cytotoxicity evaluation of photosensitizer-conjugated hexagonal upconverting nanoparticles

0571345 - ÚMCH 2024 RIV CH eng J - Journal Article
Nahorniak, Mykhailo - Oleksa, Viktoriia - Vasylyshyn, Taras - Pop-Georgievski, Ognen - Rydvalová, Eliška - Filipová, Marcela - Horák, Daniel
Cytotoxicity evaluation of photosensitizer-conjugated hexagonal upconverting nanoparticles.
Nanomaterials. Roč. 13, č. 9 (2023), č. článku 1535. E-ISSN 2079-4991
R&D Projects: GA ČR(CZ) GA21-04420S; GA MŠMT LX22NPO5102
Institutional support: RVO:61389013
Keywords : upcoverting * nanoparticles * rose bengal
OECD category: Polymer science
Impact factor: 5.3, year: 2022
Method of publishing: Open access
https://www.mdpi.com/2079-4991/13/9/1535

In this report, we synthesized hexagonal NaYF4:Yb,Er upconverting nanoparticles (UCNPs) of 171 nm in size with a narrow particle size distribution. To address their colloidal stabi-lity in aqueous media and to incorporate a photosensitizer that can produce reactive singlet oxygen (1O2) to kill tumor cells, UCNPs were conjugated with 6-bromohexanoic acid-functionalized Rose Bengal (RB) and coated with PEG-alendronate (PEG-Ale). The particles were thoroughly characterized by transmission electron microscopy, dynamic light scattering, ATR FTIR, X-ray photoelectron spectroscopy, thermogravimetric analysis, and spectrofluorometry, and 1O2 formation was detected using a 9,10-diphenylanthracene spectrophotometric probe. Cytotoxicity determination on rat mesenchymal stem cells by using the MTT assay showed that neutralization of the large positive surface charge of neat UCNPs with PEG-Ale and the bound RB sensitizer significantly reduced the concentration-dependent cytotoxicity. The presented strategy shows great potential for the use of these particles as a novel agent for the photodynamic therapy of tumors.
Permanent Link: https://hdl.handle.net/11104/0342619