Capillary isoelectric focusing-The role of markers of isoelectric point and recent applications in the field

0571276 - ÚIACH 2024 RIV NL eng J - Journal Article
Duša, Filip - Kubesová, Anna - Šalplachta, Jiří - Moravcová, Dana
Capillary isoelectric focusing-The role of markers of isoelectric point and recent applications in the field.
TrAC-Trends in Analytical Chemistry. Roč. 162, MAY (2023), č. článku 117018. ISSN 0165-9936. E-ISSN 1879-3142
R&D Projects: GA MZd(CZ) NU22-05-00110; GA MV VI20172020069; GA ČR(CZ) GA22-00236S
Institutional support: RVO:68081715
Keywords : capillary * isoelectric focusing * isoelectric point
OECD category: Analytical chemistry
Impact factor: 13.1, year: 2022
Method of publishing: Limited access

Capillary isoelectric focusing (cIEF) is a very powerful separation technique. However, successful implementation of this technique in various fields of science requires a few prerequisites including a repeatable formation of pH gradient and a set of internal standards (i.e., markers of isoelectric point) to track the actual profile of the pH gradient. Overview of the available isoelectric point markers and their syntheses is presented in this review. Moreover, recent applications of cIEF for analysis of complex larger structures such as viruses, vesicles, and bacterial cells are also reviewed.(c) 2023 Elsevier B.V. All rights reserved.
Permanent Link: https://hdl.handle.net/11104/0342538