Cryopreservation of apheresis platelets treated with riboflavin and UV light

0570648 - ÚMCH 2024 RIV GB eng J - Journal Article
Kutac, D. - Bohonek, M. - Landová, L. - Staskova, E. - Blahutova, M. - Malikova, I. - Šlouf, Miroslav - Horacek, J. M. - Stansbury, L. G. - Hess, J. R. - Seghatchian, J.
Cryopreservation of apheresis platelets treated with riboflavin and UV light.
Transfusion and Apheresis Science. Roč. 62, č. 2 (2023), č. článku 103580. ISSN 1473-0502. E-ISSN 1878-1683
Institutional support: RVO:61389013
Keywords : cryopreservation * platelets * PRT
OECD category: Polymer science
Impact factor: 1.9, year: 2022
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S1473050222002798?via%3Dihub

Pathogen reduction technology (PRT) is increasingly used in the preparation of platelets for therapeutic transfusion. As the Czech Republic considers PRT, we asked what effects PRT may have on the recovery and function of platelets after cryopreservation (CP), which we use in both military and civilian blood settings. 16 Group O apheresis platelets units were treated with PRT (Mirasol, Terumo BCT, USA) before freezing, 15 similarly collected units were frozen without PRT as controls. All units were processed with 5–6% DMSO, frozen at − 80 °C, stored > 14 days, and reconstituted in thawed AB plasma. After reconstitution, all units were assessed for: platelet count, mean platelet volume (MPV), platelet recovery, thromboelastography, thrombin generation time, endogenous thrombin potential (ETP), glucose, lactate, pH, pO2, pCO2, HCO3, CD41, CD42b, CD62, Annexin V, CCL5, CD62P, and aggregates > 2 mm and selected units for Kunicki score. PRT treated platelet units had lower platelet number (247 vs 278 ×109/U), reduced thromboelastographic MA (38 vs 62 mm) and demonstrated aggregates compared to untreated platelets. Plasma coagulation functions were largely unchanged. Samples from PRT units showed reduced platelet number, reduced function greater than the reduced number would cause, and aggregates. While the platelet numbers are sufficient to meet the European standard, marked platelets activation with weak clot strength suggest reduced effectiveness.
Permanent Link: https://hdl.handle.net/11104/0342253