Recent developments and applications of capillary and microchip electrophoresis in proteomics and peptidomics (mid-2018–2022)

0570590 - ÚOCHB 2024 RIV DE eng J - Journal Article
Štěpánová, Sille - Kašička, Václav
Recent developments and applications of capillary and microchip electrophoresis in proteomics and peptidomics (mid-2018–2022).
Journal of Separation Science. Roč. 46, č. 12 (2023), č. článku 2300043. ISSN 1615-9306. E-ISSN 1615-9314
R&D Projects: GA ČR(CZ) GA20-03899S
Institutional support: RVO:61388963
Keywords : capillary electrophoresis * mass spectrometry * peptidomics * proteomics * review
OECD category: Analytical chemistry
Impact factor: 3.1, year: 2022
Method of publishing: Open access
https://doi.org/10.1002/jssc.202300043

This review gives a wide overview of recent advances and applications of capillary electrophoresis and microchip capillary electrophoresis methods in the fields of proteomics and peptidomics in the period from mid-2018 up to the end of 2022. The methodological topics covering sample preparation and concentration techniques, hyphenation of capillary electrophoresis methods with mass spectrometry, and multidimensional separations by on-line or off-line coupled different capillary electrophoresis and liquid chromatography techniques are described and new developments in both bottom-up and top-down approaches in proteomics are presented. In addition, various applications of capillary electrophoresis methods in proteomic and peptidomic studies are demonstrated. They include monitoring of protein posttranslational modifications and applications in biological and biochemical research, clinical peptidomics and proteomics, and food analysis.
Permanent Link: https://hdl.handle.net/11104/0341890