Biological autoluminescence as a perturbance-free method for monitoring oxidation in biosystems

0569747 - ÚFE 2024 RIV GB eng J - Journal Article
Vahalová, Petra - Cifra, Michal
Biological autoluminescence as a perturbance-free method for monitoring oxidation in biosystems.
Progress in Biophysics & Molecular Biology. Roč. 177, JAN 2023 (2023), s. 80-108. ISSN 0079-6107. E-ISSN 1873-1732
R&D Projects: GA ČR(CZ) GX20-06873X
Institutional support: RVO:67985882
Keywords : Biological luminescence * Oxidative stress * Monitoring methods
OECD category: Optics (including laser optics and quantum optics)
Impact factor: 3.8, year: 2022
Method of publishing: Limited access
https://doi.org/10.1016/j.pbiomolbio.2022.10.009

Biological oxidation processes are in the core of life energetics, play an important role in cellular biophysics, physiological cell signaling or cellular pathophysiology. Understanding of biooxidation processes is also crucial for biotechnological applications. Therefore, a plethora of methods has been developed for monitoring oxidation so far, each with distinct ad-vantages and disadvantages. We review here the available methods for monitoring oxidation and their basic characteristics and capabilities. Then we focus on a unique method the only one that does not require input of additional external energy or chemicals which employs detection of biological autoluminescence (BAL). We highlight the pros and cons of this method and provide an overview of how BAL can be used to report on various aspects of cellular oxidation processes starting from oxygen consumption to the generation of oxidation products such as carbonyls. This review highlights the application potential of this completely non-invasive and label-free biophotonic diagnostic method.
Permanent Link: https://hdl.handle.net/11104/0345165