Inhibition of extracellular ice crystals growth for testing the cryodamaging effect of intracellular ice in a model of ram sperm ultra-rapid freezing

0569324 - FZÚ 2024 RIV US eng J - Journal Article
Savvulidi, F.G. - Ptáček, M. - Málková, A. - Kratochvílová, Irena - Šimek, Daniel - Martinez-Pastor, F. - Stadnik, L.
Inhibition of extracellular ice crystals growth for testing the cryodamaging effect of intracellular ice in a model of ram sperm ultra-rapid freezing.
Journal of Applied Animal Research. Roč. 51, č. 1 (2023), s. 182-192. ISSN 0971-2119. E-ISSN 0974-1844
R&D Projects: GA MŠMT(CZ) EF16_019/0000760
Grant - others:OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760
Institutional support: RVO:68378271
Keywords : cryodamage * extracellular ice * synthetic ice blocker * X-ray diffraction
OECD category: Biophysics
Impact factor: 1.4, year: 2022
Method of publishing: Open access

We aimed to test the common belief, that as the sperm cell is the smallest cell in an organism and, therefore, it contains a small amount of intracellular water, the intracellular ice should not strongly affect the sperm cell during the cryopreservation. To investigate the effect of intracellular ice, we developed a novel approach of ram spermatozoa ultra-rapid freezing with the inhibition of the growth of extracellular ice crystals during freezing. In our approach, ram sperm was supplemented with cell-impermeable synthetic ice blocker 1,4-Cyclohexanediol, leaving a sample unsupplemented as a control.
Permanent Link: https://hdl.handle.net/11104/0342071