Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study

0568944 - BC 2023 RIV CH eng J - Journal Article
Vidovic, M. - Battisti, I. - Pantelic, A. - Morina, Filis - Arrigoni, G. - Masi, A. - Jovanovic, S. V.
Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study.
Plants. Roč. 11, č. 9 (2022), č. článku 1199. E-ISSN 2223-7747
R&D Projects: GA MŠMT EF15_003/0000336
Institutional support: RVO:60077344
Keywords : cell wall remodeling * cyclic electron transport * drought * germin-like proteins * late embryogenesis abundant proteins * ojip * pectin * polyphenol oxidase * resurrection plant * superoxide dismutase
OECD category: Biochemistry and molecular biology
Impact factor: 4.5, year: 2022
Method of publishing: Open access
https://www.mdpi.com/2223-7747/11/9/1199

The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully recovers metabolic functions within one day upon watering. This study aimed to identify key candidates and pathways involved in desiccation tolerance in R. serbica. We combined differential transcriptomics and proteomics, phenolic and sugar analysis, FTIR analysis of the cell wall polymers, and detailed analysis of the photosynthetic electron transport (PET) chain. The proteomic analysis allowed the relative quantification of 1192 different protein groups, of which 408 were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost all differentially abundant proteins related to photosynthetic processes were less abundant, while chlorophyll fluorescence measurements implied shifting from linear PET to cyclic electron transport (CET). The levels of H2O2 scavenging enzymes, ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase (SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ ZnSOD isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs, mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation provoked cell wall remodeling related to GLP-derived H2O2/HO center dot activity and pectin demethylesterification. This comprehensive study contributes to understanding the role and regulation of the main metabolic pathways during desiccation aiming at crop drought tolerance improvement.
Permanent Link: https://hdl.handle.net/11104/0340330