Discovery of Long Non-Coding RNA MALAT1 Amplification in Precancerous Colorectal Lesions

0568782 - ÚEM 2023 RIV CH eng J - Journal Article
Šišková, Anna - Král, J. - Drabová, J. - Červená, Klára - Tomášová, Kristýna - Jungwirth, J. - Hucl, T. - Kohout, P. - Summerová, S. - Vodičková, Ludmila - Vodička, Pavel - Vymetálková, Veronika
Discovery of Long Non-Coding RNA MALAT1 Amplification in Precancerous Colorectal Lesions.
International Journal of Molecular Sciences. Roč. 23, č. 14 (2022), č. článku 7656. E-ISSN 1422-0067
R&D Projects: GA MZd(CZ) NV18-03-00199; GA ČR(CZ) GA22-05942S
Institutional support: RVO:68378041
Keywords : colorectal cancer * adenomas * array comparative genomic hybridization * long non-coding RNA * MALAT1
OECD category: Cell biology
Impact factor: 5.6, year: 2022
Method of publishing: Open access
https://www.mdpi.com/1422-0067/23/14/7656

A colorectal adenoma, an aberrantly growing tissue, arises from the intestinal epithelium and is considered as precursor of colorectal cancer (CRC). In this study, we investigated structural and numerical chromosomal aberrations in adenomas, hypothesizing that chromosomal instability (CIN) occurs early in adenomas. We applied array comparative genomic hybridization (aCGH) to fresh frozen colorectal adenomas and their adjacent mucosa from 16 patients who underwent colonoscopy examination. In our study, histologically similar colorectal adenomas showed wide variability in chromosomal instability. Based on the obtained results, we further stratified patients into four distinct groups. The first group showed the gain of MALAT1 and TALAM1, long non-coding RNAs (lncRNAs). The second group involved patients with numerous microdeletions. The third group consisted of patients with a disrupted karyotype. The fourth group of patients did not show any CIN in adenomas. Overall, we identified frequent losses in genes, such as TSC2, COL1A1, NOTCH1, MIR4673, and GNAS, and gene gain containing MALAT1 and TALAM1. Since long non-coding RNA MALAT1 is associated with cancer cell metastasis and migration, its gene amplification represents an important event for adenoma development.
Permanent Link: https://hdl.handle.net/11104/0340047