Mixed Infection of Blackcurrant with a Novel Cytorhabdovirus and Black Currant-Associated Nucleorhabdovirus

0568777 - BC 2023 RIV CH eng J - Journal Article
Petrzik, Karel - Přibylová, Jaroslava - Špak, Josef - Sarkisova, Tatiana - Fránová, Jana - Holub, J. - Skalík, J. - Koloniuk, Igor
Mixed Infection of Blackcurrant with a Novel Cytorhabdovirus and Black Currant-Associated Nucleorhabdovirus.
Viruses. Roč. 14, č. 11 (2022), č. článku 2456. E-ISSN 1999-4915
R&D Projects: GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) EF16_013/0001775
Grant - others:AV ČR(CZ) StrategieAV21/25; GA MŠk(CZ) LM2015042
Program: StrategieAV
Institutional support: RVO:60077344
Keywords : aphid transmission * blackcurrant rhabdovirus 2 * blackcurrant-associated rhabdovirus * electron microscopy * high throughput sequencing * Ribes nigrum
OECD category: Virology
Impact factor: 4.7, year: 2022
Method of publishing: Open access
https://www.mdpi.com/1999-4915/14/11/2456

A virome screen was performed on a new breeding line, KB1, of blackcurrant. Rhabdovirus-like particles were observed by electron microscopy in ultrathin sections of flower stalks, and the complete genome sequence of a novel virus, provisionally named blackcurrant rhabdovirus 2 (BCRV2), was determined and verified using high-throughput sequencing. The genomic organization of BCRV2 was characteristic of cytorhabdoviruses (family Rhabdoviridae) and included seven genes: 3 '-N-P '-P-P3-M-G-L-5 '. BLASTP analysis revealed that the putative L protein had the highest amino acid sequence identity (75%) with strawberry virus 2. BCRV2 was detected in Cryptomyzus galeopsidis, but efficient transmission by this aphid was not confirmed. Of note, we observed coinfection of the KB1 line with blackcurrant-associated rhabdovirus (BCaRV) by RT-PCR. This is likely the first evidence of the presence of a cyto- and a nucleorhabdovirus in a single host.
Permanent Link: https://hdl.handle.net/11104/0340042