High-resolution structural and functional deep brain imaging using holographic endo-microsocpy

0568560 - ÚPT 2023 CZ eng A - Abstract
Uhlířová, Hana - Ondráčková, Petra - Stibůrek, Miroslav - Tučková, Tereza - Turtaev, S. - Šiler, Martin - Pikálek, Tomáš - Jákl, Petr - Gomes, A. D. - Krejčí, Jana - Kolbábková, Petra - Krbková, Bára - Čižmár, Tomáš
High-resolution structural and functional deep brain imaging using holographic endo-microsocpy.
16th Multinational Congress on Microscopy, 16MCM, 04-09 September 2022, Brno, Czech Republic. Book of abstracts. Brno: Czechoslovak Microscopy Society, 2022 - (Krzyžánek, V.; Hrubanová, K.; Hozák, P.; Müllerová, I.; Šlouf, M.). s. 266-267. ISBN 978-80-11-02253-2.
[Multinational Congress on Microscopy /16./. 04.09.2022-09.09.2022, Brno]
R&D Projects: GA MŠMT EF15_003/0000476; GA MŠMT(CZ) LO1212
EU Projects: European Commission(XE) 101016787 - DEEPER
Institutional support: RVO:68081731 ; RVO:68081707
OECD category: Optics (including laser optics and quantum optics)
https://www.16mcm.cz/wp-content/uploads/2022/09/16MCM-abstract-book.pdf

Introduction: Optical microscopy present nowadays a powerful tool not just to image biological tissue but also interogate its function via optogenetics. Often, it relies on delivering the light with desired spatial and temporal distribution towards the region of interest through highly scattering tissue. For investigation of structures lying deeper than ~1.6 mm, endoscopes are used as optical relays for illumination as well as signal readout. Among different types of endoscopes, the holographic endoscopes based on multi-mode optical fibres represent the far-most atraumatic way to image in unprecedented depth with diffraction-limited resolution dictated only by the numerical aperture of the fibre and the used wavelength. Up to now, holographic endoscopes have been used in pilot imaging experiments of neurons in cultures, acute brain slices and in vivo. The first indication that spatially-resolved functional imaging of intracellular calcium may be possible to implement through the means of holographic endoscopy has been shown in Ohayon, S. et al. 2018. Results: We advance the approach of holographic endo-microscopy pushing it to its current limits using: custom-designed computer interface, computing power of the GPU, computational refocusing, novel ‘side-view’ probes and random access scanning. This enabled us to increase the resolution and field of view, image 3D volumes of the tissue without physical movement of the probe, stitch frames acquired along the path of the probe into extended fields of view and measure changes of intracellular calcium and blood flow velocity.
Permanent Link: https://hdl.handle.net/11104/0339854