Full Assessment of Lung Mechanics UsingComputer-Controlled, Forced OscillationTechnique

0568313 - ÚMG 2023 RIV US eng J - Journal Article
de Guia, Roldan M. - Žatečka, Václav - Rozman, Jan - Procházka, Jan - Sedláček, Radislav
Full Assessment of Lung Mechanics UsingComputer-Controlled, Forced OscillationTechnique.
Current Protocols. Roč. 2, č. 7 (2022), č. článku e488. ISSN 2691-1299. E-ISSN 2691-1299
R&D Projects: GA MŠMT(CZ) LM2018126
Institutional support: RVO:68378050
Keywords : endotracheal intubation * FlexiVentFX * forced oscillation technique * lung function * lung mechanics
OECD category: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Method of publishing: Open access
https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.488

The forced oscillation technique (FOT) is a powerful and accurate methodto quantify the mechanical properties of the airways and tissues of the res-piratory system. Here we provide a detailed protocol for the measurement ofmouse respiratory mechanical parameters. We present a procedure for mouseendotracheal intubation using a handcrafted intubation platform and confir-mation module. The FlexiVentFXTMsystem (Scireq Inc.) is utilized for thethorough assessment of lung function with the FlexiWareTMsoftware serv-ing as a unit for the planning, experimentation, and analysis. The protocol hasbeen standardized and adapted for use by our center for lung-function pheno-typing of mouse models generated for the International Mouse PhenotypingConsortium (IMPC). The simplified steps, technical considerations, and inte-grated hardware-software demonstration make this protocol adaptable and im-plementable for researchers interested in using FOT for lung-function evaluation
Permanent Link: https://hdl.handle.net/11104/0339641