Collective behavior of magnetic microrobots through immuno-sandwich assay: On-the-fly COVID-19 sensing

0568185 - BC 2023 RIV NL eng J - Journal Article
Mayorga-Martinez, C. C. - Vyskočil, J. - Novotný, F. - Bednář, P. - Růžek, Daniel - Alduhaish, O. - Pumera, M.
Collective behavior of magnetic microrobots through immuno-sandwich assay: On-the-fly COVID-19 sensing.
Applied Materials Today. Roč. 26, MAR (2022), č. článku 101337. ISSN 2352-9407. E-ISSN 2352-9407
Institutional support: RVO:60077344
Keywords : Microrobots * Biosensor * Covid19
OECD category: Textiles
Impact factor: 8.3, year: 2022
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S2352940721003875?via%3Dihub

Mobile self-propelled micro/nanorobots are mobile binding surface that improved the sensitivity of many biosensing system by on-the-fly identification and isolation of different biotargets. Proteins are powerful tools to predict infectious disease progression such as COVID-19. The main methodology used to COVID-19 detection is based on ELISA test by antibodies detection assays targeting SARS-CoV-2 virus spike protein and nucleocapside protein that represent an indirect SARS-CoV-2 detection with low sentitivy and specificity. Moreover ELISA test are limited to used external shaker to obtain homogenously immobilization of antibodies and protein on sensing platform. Here, we present magnetic microrobots that collective self-assembly through immuno-sandwich assay and they can be used as mobile platform to detect on-the-fly SARS-CoV-2 virus particle by its spike protein. The collective self-assembly of magnetic microrobots through immuno-sandwich assay enhanced its analytical performance in terms of sensitivity decreasing the detection limit of SARS-CoV-2 virus by one order of magnitude with respect to the devices previously reported. This proof-of-concept of microrobotics offer new ways to the detection of viruses and proteins of medical interest in general.(c) 2021 Elsevier Ltd. All rights reserved.
Permanent Link: https://hdl.handle.net/11104/0339518