Development of a portable hypoxia chamber for ultra-high dose rate laser-driven proton radiobiology applications

0568030 - FZÚ 2023 RIV GB eng J - Journal Article
Chaudhary, P. - Gwynne, D. - Odložilík, Boris - McMurray, A. - Milluzzo, G. - Maiorino, C. - Doria, D. - Ahmed, H. - Romagnani, L. - Alejo, A. - Padda, H. - Green, J. - Carroll, D. - Booth, N. - McKenna, P. - Kar, S. - Petringa, G. - Catalano, R. - Cammarata, F.P. - Cirrone, G. A. P. - McMahon, S. J. - Prise, K. M. - Borghesi, M.
Development of a portable hypoxia chamber for ultra-high dose rate laser-driven proton radiobiology applications.
Radiation Oncology. Roč. 17, č. 1 (2022), č. článku 77. E-ISSN 1748-717X
EU Projects: European Commission(XE) 18HLT04 - UHDpulse
Institutional support: RVO:68378271
Keywords : ultra-high dose rate * laser-driven protons * hypoxia * DNA repair
OECD category: Particles and field physics
Impact factor: 3.6, year: 2022
Method of publishing: Open access

Oxygen measurements showed that our chambers maintained a radiobiological hypoxic environment for at least 45 min and pathological hypoxia for up to 24 h after disconnecting the chambers from the gas supply. We observed a significant reduction in the 53BP1 foci induced by laser-driven protons, conventional protons and X-rays in the hypoxic cells compared to normoxic cells at 30 min post-irradiation. Under hypoxic irradiations, the Laser-driven protons induced significant residual DNA DSB damage in hypoxic AG01522B cells compared to the conventional dose rate protons suggesting an important impact of these extremely high dose-rate exposures. We obtained an oxygen enhancement ratio (OER) of 2.1 ± 0.1 and 2.5 ± 0.1 respectively for the AG01522B and patient-derived GBM stem cells for X-ray irradiation using our hypoxia chambers.
Permanent Link: https://hdl.handle.net/11104/0339379