Resolving measurement of large (~ GDa) chemical/biomolecule complexes with multimode nanomechanical resonators

0567870 - FZÚ 2023 RIV CH eng J - Journal Article
Stachiv, Ivo - Machů, Zdeněk - Ševeček, O. - Tuhovcak, O. - Kotoul, M. - Jeng, Y.-R.
Resolving measurement of large (~ GDa) chemical/biomolecule complexes with multimode nanomechanical resonators.
Sensors and Actuators B - Chemical. Roč. 353, Feb (2022), č. článku 131062. E-ISSN 0925-4005
R&D Projects: GA ČR(CZ) GC21-12994J; GA MŠMT(CZ) EF16_019/0000760
Grant - others:OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760
Institutional support: RVO:68378271
Keywords : mass sensing * mass spectrometry * nanomechanical resonators * molecule identification * heavy mass (similar to GDa) spectrometry
OECD category: Condensed matter physics (including formerly solid state physics, supercond.)
Impact factor: 8.4, year: 2022
Method of publishing: Limited access
https://doi.org/10.1016/j.snb.2021.131062

Mass sensing by nanomechanical resonators can be routinely performed for analytes of mass ranging from kDa to tens of MDa. Measurement of the heavier analytes (up to hundreds of GDa) that are relevant to viruses, and many biological and chemical complexes, still remains one of the main challenges to be solved. Some studies propose the heavy analyte identification by accounting for its mass, stiffness and binding effects. However, the necessity of using the sophisticated computational tools complicates their widespread use in the nanomechanical mass spectrometry. Here, we report on the heavy analyte mass spectrometry (~ GDa) using the multimode nanomechanical resonators, which is directly applicable to analytes of arbitrary mass, stiffness and dimensions. This identification, based on the simultaneous measurement of the multiple by analyte induced resonant frequency shifts, only requires the analyte to resonator mass ratio between 0.001 and 0.02.
Permanent Link: https://hdl.handle.net/11104/0339126