The co-culture of keratinocytes and fibroblasts on a multi-layered polyester nanofibrous membrane enriched with platelet lysate

0567464 - FGÚ 2023 RIV CZ eng C - Conference Paper (international conference)
Blanquer, Andreu - Filová, Elena - Jenčová, V. - Brož, Antonín - Kuželová Košťáková, E. - Lisnenko, M. - Procházková, R. - Bačáková, Lucie
The co-culture of keratinocytes and fibroblasts on a multi-layered polyester nanofibrous membrane enriched with platelet lysate.
NANOCON 2021 - Conference proceedings. Ostrava: Tanger Ltd., 2021, s. 234-239. ISBN 978-80-88365-00-6. ISSN 2694-930X.
[International Conference on Nanomaterials - Research & Application /13./ NANOCON. Brno (CZ), 20.10.2021-22.10.2021]
R&D Projects: GA MZd(CZ) NV18-01-00332
EU Projects: European Commission(XE) 101003407 - ELECTROSKIN
Institutional support: RVO:67985823
Keywords : skin wound healing * electrospun nanofibers * platelet lysate * keratinocytes * fibroblasts
OECD category: Endocrinology and metabolism (including diabetes, hormones)
https://www.confer.cz/nanocon/2021/read/4364-the-co-culture-of-keratinocytes-and-fibroblasts-on-a-multi-layered-polyester-nanofibrous-membrane-enriched-with-platelet-lysate.pdf

The prevalence of chronic wounds is increasing due to the population ageing and specific illnesses like diabetes mellitus and vascular diseases. Nanofibrous membranes fabricated using synthetic polymers are promising materials to enhance skin wound healing. PCL and PVA membranes are being studied to be used as scaffolds for skin tissue engineering and hydrogels for controlled drug delivery, respectively. The present study considers the development of a multi-layered membrane made of PCL and PVA loaded with platelet lysate (PL). PCL nanofibers allowed cell adhesion and growth, whereas PVA acted as a hydrogel that releases the bioactive compounds of platelet lysate. The cytocompatibility of the membranes containing PL and without it was demonstrated on two cell types involved in wound healing, i.e. keratinocytes and fibroblasts. Both cell types were able to adhere and proliferate on the membranes. In addition, the membrane containing PL enhanced the proliferation of fibroblasts. A co-culture study was also performed by seeding each cell type on one side of the membrane. The cells were co-cultured for 7 days and the results showed that PL increased the proliferation of cells achieving a monolayer of keratinocytes or fibroblasts on each side of the membrane. Thus, the beneficial effect of PCL-PVA+PL membranes on monocultures and co-cultures of skin cells was demonstrated, and these membranes can be considered potential scaffolds for treatment of chronic wounds.
Permanent Link: https://hdl.handle.net/11104/0338714