Gene polymorphisms and serum levels of mannose-binding lectin in Czech patients with recurrent aphthous stomatitis: A case-control study

0566876 - MBÚ 2023 RIV US eng J - Journal Article
Izakovičová, P. - Slezaková, S. - Jirásková Zákostelská, Zuzana - Šístková, J. - Mlčuchová, N. - Bartová, J. - Petanová, J. - Kukíinek, P. - Fassmann, A. - Dušek, L. - Hollá, L. I. - Linhartová, P. B.
Gene polymorphisms and serum levels of mannose-binding lectin in Czech patients with recurrent aphthous stomatitis: A case-control study.
Journal of Oral Pathology & Medicine. Roč. 52, č. 1 (2023), s. 81-90. ISSN 0904-2512. E-ISSN 1600-0714
R&D Projects: GA MZd(CZ) NV15-29336A
Research Infrastructure: RECETOX RI - 90121
Institutional support: RVO:61388971
Keywords : haplogenotype * haplotype * mbl * polymorphism * recurrent aphthous stomatitis
OECD category: Dentistry, oral surgery and medicine
Impact factor: 3.3, year: 2022
Method of publishing: Open access
https://onlinelibrary.wiley.com/doi/10.1111/jop.13385

Background: Recurrent aphthous stomatitis is one of the most prevalent oral mucosal immunological diseases. A recent case-control study in the Egyptian population suggested that single nucleotide polymorphism Gly54Asp (rs1800450) of the mannose-binding lectin 2 gene might affect the mannose-binding lectin serum level and recurrent aphthous stomatitis development. The aim of this study was to determine the distribution of six functional mannose-binding lectin 2 gene polymorphisms and analyse their role in recurrent aphthous stomatitis susceptibility in the Czech population. Methods: The study included 227 subjects, 137 healthy people and 90 patients with recurrent aphthous stomatitis. Six mannose-binding lectin 2 gene polymorphisms (rs11003125, rs7096206, rs7095891, rs5030737, rs1800450, rs1800451) were analysed by the SNaPshot assay method, mannose-binding lectin serum levels were determined by enzyme-linked immunosorbent assay (ELISA) method in a subgroup of subjects (N = 87). Results: No significant differences in mean of mannose-binding lectin serum levels between healthy controls and patients with recurrent aphthous stomatitis were observed (383 ng/ml +/- 249 standard deviation (SD) vs. 316 ng/ml +/- 177 SD in remission phase vs. 343 ng/ml +/- 254 SD in active phase, p > 0.05), also the allele and genotype frequencies of the studied mannose-binding lectin 2 polymorphisms did not differ significantly between the two groups (p > 0.05, odds ratio (OR): 0.75-1.23). Moreover, the distribution of mannose-binding lectin 2 haplotypes and haplogenotypes was similar in the healthy subjects and patients with recurrent aphthous stomatitis (p > 0.05, OR: 0.75-1.23). Conclusions: This study did not confirm the previously reported association of the mannose-binding lectin 2 Gly54Asp gene variant and low mannose-binding lectin serum level as the risk factors for susceptibility to recurrent aphthous stomatitis. In addition, no significant relationships between mannose-binding lectin 2 functional haplotypes or haplogenotypes and recurrent aphthous stomatitis were observed.
Permanent Link: https://hdl.handle.net/11104/0338151