Diffusive dynamics of bacterial proteome as a proxy of cell death

0566839 - ÚFCH JH 2024 RIV US eng J - Journal Article
Di Barri, D. - Timr, Štěpán - Guiral, M. - Giudici-Orticoni, M.-T. - Seydel, T. - Beck, Ch. - Petrillo, C. - Derreumaux, P. - Melchionna, S. - Sterpone, F. - Peters, J. - Paciaroni, A.
Diffusive dynamics of bacterial proteome as a proxy of cell death.
ACS Central Science. Roč. 9, č. 1 (2023), s. 93-102. ISSN 2374-7943. E-ISSN 2374-7951
EU Projects: European Commission(XE) 840395
Institutional support: RVO:61388955
Keywords : Bacteria * Diffusion * Transport properties
OECD category: Physical chemistry
Impact factor: 18.2, year: 2022
Method of publishing: Open access

Temperature variations have a big impact on bacterial metabolism and death, yet an exhaustive molecular picture of these processes is still missing. For instance, whether thermal death is determined by the deterioration of the whole or a specific part of the proteome is hotly debated. Here, by monitoring the proteome dynamics of E. coli, we clearly show that only a minor fraction of the proteome unfolds at the cell death. First, we prove that the dynamical state of the E. coli proteome is an excellent proxy for temperature-dependent bacterial metabolism and death. The proteome diffusive dynamics peaks at about the bacterial optimal growth temperature, then a dramatic dynamical slowdown is observed that starts just below the cell’s death temperature. Next, we show that this slowdown is caused by the unfolding of just a small fraction of proteins that establish an entangling interprotein network, dominated by hydrophobic interactions, across the cytoplasm. Finally, the deduced progress of the proteome unfolding and its diffusive dynamics are both key to correctly reproduce the E. coli growth rate.
Permanent Link: https://hdl.handle.net/11104/0338111