Plant material selection, collection, preservation, and storage for nuclear DNA content estimation

0566766 - BÚ 2023 RIV US eng J - Journal Article
Čertner, Martin - Lučanová, Magdalena - Sliwinska, E. - Kolář, Filip - Loureiro, J.
Plant material selection, collection, preservation, and storage for nuclear DNA content estimation.
Cytometry. Part A. Roč. 101, č. 9 (2022), s. 737-748. ISSN 1552-4922. E-ISSN 1552-4930
Institutional support: RVO:67985939
Keywords : flow cytometry * nuclear DNA content * nuclear suspensions
OECD category: Plant sciences, botany
Impact factor: 3.7, year: 2022
Method of publishing: Limited access
https://doi.org/10.1002/cyto.a.24482

In theory, any plant tissue providing intact nuclei in sufficient quantity is suitable for nuclear DNA content estimation using flow cytometry (FCM). While this certainly opens a wide variety of possible applications of FCM, especially when compared to classical karyological techniques restricted to tissues with active cell division, tissue selection and quality may directly affect the precision (and sometimes even reliability) of FCM measurements. It is usually convenient to first consider the goals of the study to either aim for the highest possible accuracy of estimates (e.g., for inferring genome size, detecting homoploid intraspecific genome size variation, aneuploidy, among others), or to decide that histograms of reasonable resolution provide sufficient information (e.g., ploidy level screening within a single model species). Here, a set of best practices guidelines for selecting the optimal plant tissue for FCM analysis, sampling of material, and material preservation and storage are provided. In addition, factors potentially compromising the quality of FCM estimates of nuclear DNA content and data interpretation are discussed.
Permanent Link: https://hdl.handle.net/11104/0338057