2D and 3D cartilage models for drug testing and high throughput screening – the impact of PORCN inhibition on cartilage development

0566666 - ÚIACH 2023 CH eng C - Conference Paper (international conference)
Killinger, Michael - Szotkowská, Tereza - Buchtová, Marcela - Klepárník, Karel
2D and 3D cartilage models for drug testing and high throughput screening – the impact of PORCN inhibition on cartilage development.
NanoBioTech-Montreux Conference 2022. The NanoBiotech Association, 2022, s. 123-124.
[Annual European Conference on Micro & Nanoscale Technologies for the Life Sciences /25./. Montreux (CH), 14.11.2022-16.11.2022]
R&D Projects: GA ČR(CZ) GA20-00726S
Institutional support: RVO:68081715 ; RVO:67985904
Keywords : cartilage regeneration * Wnt signaling * microfluidics
OECD category: Analytical chemistry; Developmental biology (UZFG-Y)

In this study, several in vitro 2D and 3D methods were established to study the role of PORCN in chondrogenesis in regenerative medicine. Here, we used classical animal models such as primary cell cultures, tissue cultures of long bones, and modern 3D cell culture approaches. Modifying the PORCN function with the LGK-974 inhibitor showed a massive increase in cartilage mass in all model systems. 3D cell cultures were compared to standard culture methods to reflect the benefits of microfluidic platforms. Furthermore, our PDMS platform base can be connected to the syringe pump to reproduce in vivo perfusion conditions and increase the value of 3D cell culture.
Permanent Link: https://hdl.handle.net/11104/0337979