An autoactive NB-LRR gene causes Rht13 dwarfism in wheat

0566545 - ÚEB 2024 RIV US eng J - Journal Article
Borrill, P. - Mago, R. - Xu, T. - Ford, B. A. - Williams, S. J. - Derkx, A. - Bovill, W. D. - Hyles, J. - Bhatt, D. - Xia, X. - MacMillan, C. - White, R. - Buss, W. - Molnár, István - Walkowiak, S. - Olsen, O.A. - Doležel, Jaroslav - Pozniak, C. - Spielmeyer, W.
An autoactive NB-LRR gene causes Rht13 dwarfism in wheat.
Proceedings of the National Academy of Sciences of the United States of America. Roč. 119, č. 48 (2022), č. článku e2209875119. ISSN 0027-8424. E-ISSN 1091-6490
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : autoactive NB-LRR * reduced-height (Rht) gene * semidwarfing gene * Triticum aestivum L. (wheat)
OECD category: Biochemistry and molecular biology
Impact factor: 11.1, year: 2022
Method of publishing: Open access
https://doi.org/10.1073/pnas.2209875119

Semidwarfing genes have greatly increased wheat yields globally, yet the widely used gibberellin (GA)-insensitive genes Rht-B1b and Rht-D1b have disadvantages for seedling emergence. Use of the GA-sensitive semidwarfing gene Rht13 avoids this pleiotropic effect. Here, we show that Rht13 encodes a nucleotide-binding site/leucine-rich repeat (NB-LRR) gene. A point mutation in the semidwarf Rht-B13b allele autoactivates the NB-LRR gene and causes a height reduction comparable with Rht-B1b and Rht-D1b in diverse genetic backgrounds. The autoactive Rht-B13b allele leads to transcriptional up-regulation of pathogenesis-related genes including class III peroxidases associated with cell wall remodeling. Rht13 represents a new class of reduced height (Rht) gene, unlike other Rht genes, which encode components of the GA signaling or metabolic pathways. This discovery opens avenues to use autoactive NB-LRR genes as semidwarfing genes in a range of crop species, and to apply Rht13 in wheat breeding programs using a perfect genetic marker.
Permanent Link: https://hdl.handle.net/11104/0337870