Rolling circle amplification tailored for plasmonic biosensors: from ensemble to single-molecule detection

0566261 - FZÚ 2023 RIV US eng J - Journal Article
Schmidt, K. - Hageneder, S. - Lechner, B. - Zbiral, B. - Fossati, S. - Ahmadi, Y. - Minunni, M. - Toca-Herrera, J.L. - Reimhult, E. - Barisic, I. - Dostálek, Jakub
Rolling circle amplification tailored for plasmonic biosensors: from ensemble to single-molecule detection.
ACS Applied Materials and Interfaces. Roč. 14, č. 49 (2022), s. 55017-55027. ISSN 1944-8244. E-ISSN 1944-8252
Institutional support: RVO:68378271
Keywords : rolling circle amplification * surface plasmon resonance * surface plasmon-enhanced fluorescence * biosensor * single molecule immunoassays
OECD category: Biophysics
Impact factor: 9.5, year: 2022
Method of publishing: Open access

We report on the tailoring of rolling circle amplification (RCA) for affinity biosensors relying on the optical probing of their surface with confined surface plasmon field. Affinity capture of the target analyte at the metallic sensor surface (e.g., by using immunoassays) is followed by the RCA step for subsequent readout based on increased refractive index (surface plasmon resonance, SPR) or RCA-incorporated high number of fluorophores (in surface plasmon-enhanced fluorescence, PEF). By combining SPR and PEF methods, this work investigates the impact of the conformation of long RCA-generated single-stranded DNA (ssDNA) chains to the plasmonic sensor response enhancement. In order to confine the RCA reaction within the evanescent surface plasmon field and hence maximize the sensor response, an interface carrying analyte-capturing molecules and additional guiding ssDNA strands (complementary to the repeating segments of RCA-generated chains) is developed.
Permanent Link: https://hdl.handle.net/11104/0337647