Characterization of insulin crystalline form in isolated β-cell secretory granules

0566032 - ÚOCHB 2023 RIV GB eng J - Journal Article
Asai, Seiya - Moravcová, J. - Žáková, Lenka - Selicharová, Irena - Hadravová, Romana - Brzozowski, A. M. - Nováček, J. - Jiráček, Jiří
Characterization of insulin crystalline form in isolated β-cell secretory granules.
Open Biology. Roč. 12, č. 12 (2022), č. článku 220322. E-ISSN 2046-2441
R&D Projects: GA MŠMT(CZ) EF16_019/0000729
Research Infrastructure: CIISB II - 90127
Institutional support: RVO:61388963
Keywords : insulin secretory granules * protein crystals * in vivo crystalization * cryo-EM
OECD category: Cell biology
Impact factor: 5.8, year: 2022
Method of publishing: Open access
https://doi.org/10.1098/rsob.220322

Insulin is stored in vivo inside the pancreatic β-cell insulin secretory granules. However, the structural features of the storage forms of insulin in native preparations of secretory granules are unknown, because of their small size, fragile character and difficult handling. We isolated and investigated the secretory granules from MIN6 cells under near-native conditions, using cryo-electron microscopic (Cryo-EM) techniques. Our experimental data show that insulin can be pre-sent in pancreatic MIN6 cell granules in a microcrystalline form, probably consisting of 4Zn2+-hexamers of this hormone
Permanent Link: https://hdl.handle.net/11104/0337471