Protein-induced transformation of unilamellar to multilamellar vesicles triggered by a polysaccharide

0565998 - ÚMCH 2024 RIV GB eng J - Journal Article
Papagiannopoulos, A. - Sklapani, A. - Len, A. - Radulescu, A. - Pavlova, Ewa - Šlouf, Miroslav
Protein-induced transformation of unilamellar to multilamellar vesicles triggered by a polysaccharide.
Carbohydrate Polymers. Roč. 303, 1 March (2023), č. článku 120478. ISSN 0144-8617. E-ISSN 1879-1344
Institutional support: RVO:61389013
Keywords : didodecyldimethylammonium bromide * bovine serum albumin * hyaluronic acid
OECD category: Polymer science
Impact factor: 11.2, year: 2022
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0144861722013832?via%3Dihub

We report on the morphological transitions of didodecyldimethylammonium bromide (DDAB) cationic vesicles and hybrid DDAB/hyaluronic acid (HA) vesicles upon addition of BSA at pH 7 where BSA is overall negatively charged. Small angle neutron scattering (SANS) is used to extract the size distributions of the nanovesicles, the thickness of the DDAB bilayers and their lamellarity. Although the HA-decorated DDAB vesicles contain the negatively charged polysaccharide the interaction with BSA appears to be more intense in comparison to bare vesicles. Characteristic peaks in the SANS patterns indicate the presence of multilamellar interfaces while the formation of multilamellar vesicles induced by BSA depends on the amount of added HA. Consequently, higher lamellarities are observed at higher BSA contents. This work demonstrates a simple methodology to tune the encapsulation of globular proteins in vesicular nanoassemblies by affecting their lamellarity and has direct implications on the application of vesicles and liposomes in protein delivery.
Permanent Link: https://hdl.handle.net/11104/0337457