Expression profiling and cellular localization of myxozoan minicollagens during nematocyst formation and sporogenesis

0565966 - BC 2023 RIV AU eng J - Journal Article
Kyslík, Jiří - Vancová, Marie - Bartošová-Sojková, Pavla - Lövy, Alena - Holzer, Astrid S. - Fiala, Ivan
Expression profiling and cellular localization of myxozoan minicollagens during nematocyst formation and sporogenesis.
International Journal for Parasitology. Roč. 52, č. 10 (2022), s. 667-675. ISSN 0020-7519. E-ISSN 1879-0135
R&D Projects: GA ČR(CZ) GX19-28399X; GA MŠMT(CZ) LM2018129; GA MŠMT(CZ) EF18_046/0016045
Institutional support: RVO:60077344
Keywords : Cnidaria * Myxozoa * Parasite * Ncol-5 * Nematocyst * Quantitative PCR * Immunolabelling
OECD category: Cell biology
Impact factor: 4, year: 2022
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S0020751922001047?via%3Dihub

In free-living cnidarians, minicollagens are major structural components in the biogenesis of nemato-cysts. Recent sequence mining and proteomic analysis demonstrate that minicollagens are also expressed by myxozoans, a group of evolutionarily ancient cnidarian endoparasites. Nonetheless, the presence and abundance of nematocyst-associated genes/proteins in nematocyst morphogenesis have never been studied in Myxozoa. Here, we report the gene expression profiles of three myxozoan minicollagens, ncol-1, ncol-3, and the recently identified noncanonical ncol-5, during the intrapiscine development of Myxidium lieberkuehni, the myxozoan parasite of the northern pike, Esox lucius. Moreover, we localized the myxozoan-specific minicollagen Ncol-5 in the developing myxosporean stages by Western blotting, immunofluorescence, and immunogold electron microscopy. We found that expression of minicollagens was spatiotemporally restricted to developing nematocysts within the myxospores during sporogenesis. Intriguingly, Ncol-5 is localized in the walls of nematocysts and predominantly in nematocyst tubules. Overall, we demonstrate that despite being significantly reduced in morphology, myxozoans retain struc-tural components associated with nematocyst development in free-living cnidarians. Furthermore, our findings have practical implications for future functional and comparative studies as minicollagens are useful markers of the developmental phase of myxozoan parasites.(c) 2022 The Author(s). Published by Elsevier Ltd on behalf of Australian Society for Parasitology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Permanent Link: https://hdl.handle.net/11104/0339405