Structure-based insights into evolution of rhodopsins

0565888 - FZÚ 2023 RIV GB eng J - Journal Article
Zabelskii, D. - Dmitrieva, N. - Volkov, O. - Shevchenko, V. - Kovalev, K. - Balandin, T. - Soloviov, D. V. - Astashkin, A. - Zinovev, E. - Alekseev, A. - Round, E. - Polovinkin, Vitaly - Chizhov, I. - Rogachev, A. - Okhrimenko, I. - Borshchevskiy, V. - Chupin, V. - Buldt, G. - Yutin, N. - Bamberg, E. - Koonin, E. - Gordeliy, V.
Structure-based insights into evolution of rhodopsins.
Communications Biology. Roč. 4, č. 1 (2021), č. článku 821. E-ISSN 2399-3642
Institutional support: RVO:68378271
Keywords : driven proton pump * lipidic cubic phases * sensory rhodopsin * crystallographic structure
OECD category: Particles and field physics
Impact factor: 6.548, year: 2021
Method of publishing: Open access

Rhodopsins, most of which are proton pumps generating transmembrane electrochemical proton gradients, span all three domains of life, are abundant in the biosphere, and could play a crucial role in the early evolution of life on earth. Whereas archaeal and bacterial proton pumps are among the best structurally characterized proteins, rhodopsins from unicellular eukaryotes have not been well characterized. To fill this gap in the current understanding of the proton pumps and to gain insight into the evolution of rhodopsins using a structure-based approach, we performed a structural and functional analysis of the light-driven proton pump LR (Mac) from the pathogenic fungus Leptosphaeria maculans. The first high-resolution structure of fungi rhodopsin and its functional properties reveal the striking similarity of its membrane part to archaeal but not to bacterial rhodopsins.

Permanent Link: https://hdl.handle.net/11104/0337367


Research data: Protein Data Bank