Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays

0565880 - ÚIACH 2023 RIV US eng J - Journal Article
Makhneva, E. - Sklenárová, D. - Brandmeier, J. C. - Hlaváček, Antonín - Gorris, H. H. - Skládal, P. - Farka, Z.
Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays.
Analytical Chemistry. Roč. 94, č. 47 (2022), s. 16376-16383. ISSN 0003-2700. E-ISSN 1520-6882
R&D Projects: GA ČR(CZ) GA21-03156S
Institutional support: RVO:68081715
Keywords : assays * fluorescence * up-conversion nanoparticles * immunosensor
OECD category: Analytical chemistry
Impact factor: 7.4, year: 2022
Method of publishing: Limited access

Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels-horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)-UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection.
Permanent Link: https://hdl.handle.net/11104/0337362