Cathodoluminescence imaging of cellular structures labeled with luminescent iridium or rhenium complexes at cryogenic temperatures

0565268 - BC 2023 RIV DE eng J - Journal Article
Vancová, Marie - Skoupý, Radim - Ďurinová, Eva - Bílý, Tomáš - Nebesářová, Jana - Krzyžánek, Vladislav - Kolouch, A. - Horodysky, P.
Cathodoluminescence imaging of cellular structures labeled with luminescent iridium or rhenium complexes at cryogenic temperatures.
Scientific Reports. Roč. 12, č. 1 (2022), č. článku 13432. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA MŠMT(CZ) LM2018129; GA TA ČR(CZ) TN01000008
Institutional support: RVO:60077344 ; RVO:68081731
Keywords : Cathodoluminescence * cellular * cryogenic
OECD category: Cell biology
Impact factor: 4.6, year: 2022
Method of publishing: Open access
https://www.nature.com/articles/s41598-022-17723-w

We report for the first time the use of two live-cell imaging agents from the group of luminescent transition metal complexes (IRAZOLVE-MITO and REZOLVE-ER) as cathodoluminescent probes. This first experimental demonstration shows the application of both probes for the identification of cellular structures at the nanoscale and near the native state directly in the cryo-scanning electron microscope. This approach can potentially be applied to correlative and multimodal approaches and used to target specific regions within vitrified samples at low electron beam energies.
Permanent Link: https://hdl.handle.net/11104/0336839